FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13913700
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC148750.073.15937120
GAGCACA229400.042.861855120
AGAGCAC310150.034.299946120
AAGAGCA481800.022.504845120
GAAGAGC527500.020.374481118-119
GGAAGAG643950.017.22768120
TCGGAAG747650.014.793998118-119
CGGAAGA768250.014.474688120
ATCGGAA873250.012.462304118-119
GATCGGA892850.012.4473116-117
AGATCGG993600.010.929667116-117
TAGATCG202950.010.315226114-115
CTAGATC56450.09.709209120
AAGATCG477300.09.269475114-115
TTAGATC100750.08.310711110-111
GAGATCG344400.07.9187493120
TAAGATC187150.07.679702108-109
CAGATCG313000.07.2044888118-119
CAAGATC268100.07.0830116112-113
TGAGATC148900.06.553741112-113
Produced by FastQC (version 0.11.5)