FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16705554
Sequences flagged as poor quality0
Sequence length64-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG88200.015.850063120
ATCTCGG934600.09.218757120
TGGTCGC534050.09.088878120
GTGGTCG585400.08.886077120
CTCGGTG807550.08.566503120
TCGGTGG759350.08.454452120
GGTGGTC639450.08.303866120
CGGTGGT721050.08.254484120
CGCCGTA437300.08.218471120
TCGCCGT492250.08.203006120
TAGATCT1111700.08.181921120
GGTCGCC507500.08.157494118-119
AAAAAAA1114200.08.120482120
GTCGCCG513250.08.077794120
AGATCTC1059800.08.03345120
GATCTCG1012650.07.9512763120
TCTCGGT856350.07.9241915120
GCCGTAT435150.07.7075524120
CGTATCA360550.07.588274120
CCGTATC438250.07.557198120
Produced by FastQC (version 0.11.5)