FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16705554
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC219750.082.682625120
GAGCACA342750.052.03923120
AGAGCAC475100.038.541855120
AAGAGCA719900.025.470398118-119
GAAGAGC795250.023.021433118-119
GGAAGAG1015450.018.681637120
CGGAAGA1189150.015.580435118-119
TCGGAAG1199750.015.328605114-115
ATCGGAA1422900.013.098045120
GATCGGA1453250.012.753869118-119
CACGTCT62600.012.678527120
AGATCGG1573400.011.848276114-115
CACACGT62100.011.297145120
TAGATCG398800.010.96365120
ACACGTC68250.010.071557118-119
CTAGATC89300.010.039445110-111
AAGATCG613300.09.561908116-117
TTAGATC152150.09.408209118-119
GAGATCG483350.09.178435114-115
CAGATCG509100.08.818598114-115
Produced by FastQC (version 0.11.5)