FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13143667
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC121800.073.58513120
GAGCACA189900.046.883522120
AGAGCAC265200.034.094963120
AAGAGCA406150.022.205343118-119
GAAGAGC485600.019.232738120
GGAAGAG598100.015.570941120
TCGGAAG679600.014.005117120
CGGAAGA705600.013.33753118-119
ATCGGAA788150.012.579404120
GATCGGA786250.012.374418120
AGATCGG861300.011.332138118-119
TAGATCG202100.010.694466120
CTAGATC46850.010.482664112-113
TTAGATC74500.09.581756120
CCTAGAT18000.09.1558114-115
TCTAGAT22500.08.789301110-111
CCCTAGA5751.1207467E-68.59849114-115
AAGATCG335300.08.542629116-117
GAGATCG343600.08.470575116-117
ACTAGAT16650.07.720332110-111
Produced by FastQC (version 0.11.5)