FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12697077
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC153200.069.56149120
GAGCACA240050.044.917427120
AGAGCAC319750.035.520187120
AAGAGCA494350.023.723661120
GAAGAGC544550.021.275475118-119
GGAAGAG671950.017.778038120
CGGAAGA802500.014.6013565118-119
TCGGAAG808800.014.52027118-119
ATCGGAA957850.012.61657120
GATCGGA973200.012.431158120
AGATCGG1073100.011.276184118-119
TAGATCG254900.010.760113114-115
ACACGTC48900.010.680236120
CACACGT46200.010.302749120
CTAGATC66350.09.963723120
CACGTCT43450.09.58547120
GAGATCG392000.09.09003120
TTAGATC96650.08.823694120
AAGATCG418850.08.644861118-119
CCTAGAT22050.08.232652114-115
Produced by FastQC (version 0.11.5)