FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12035361
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC135650.070.9959120
GAGCACA213600.046.831955120
AGAGCAC280200.035.084606118-119
AAGAGCA437200.022.84994120
GAAGAGC483450.020.526297120
GGAAGAG594850.017.017914120
TCGGAAG722050.014.923274120
CGGAAGA713100.014.793242120
GATCGGA850350.012.804721120
ATCGGAA846400.012.798221118-119
AGATCGG935000.011.560941116-117
TAGATCG244200.011.365144120
CTAGATC56800.011.014982118-119
TTAGATC93500.09.681876114-115
ACACGTC47750.09.1993065120
CACACGT44050.09.06546120
GAGATCG355550.08.882593118-119
AAGATCG318900.08.702942120
CAGATCG307950.08.429244118-119
CGCTAGA6405.066049E-78.320105116-117
Produced by FastQC (version 0.11.5)