FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12590166
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC133650.062.65038120
GAGCACA206400.042.086872120
AGAGCAC272650.032.435295120
AAGAGCA430300.021.265299120
GAAGAGC482950.019.731394120
GGAAGAG595850.016.168129120
TCGGAAG674700.014.371023118-119
CGGAAGA694150.014.18903120
ATCGGAA808650.012.664561120
GATCGGA801550.012.150179116-117
AGATCGG884900.011.735662120
TAGATCG213200.010.752875120
CTAGATC54800.010.071505118-119
TTAGATC84750.09.247966120
AAGATCG406600.08.762855118-119
GAGATCG295250.08.428558118-119
TAAGATC162250.07.8298445118-119
CAGATCG280700.07.4690833120
ACGTCTG55250.07.447541120
ACACGTC41150.07.3014016118-119
Produced by FastQC (version 0.11.5)