FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12652525
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC166300.088.41681120
GAGCACA280100.053.721527120
AGAGCAC372700.039.956436118-119
AAGAGCA551700.027.181782118-119
GAAGAGC626650.023.781368116-117
GGAAGAG747500.020.12617116-117
CGGAAGA877800.017.065569120
TCGGAAG899450.016.646011114-115
ATCGGAA1050800.014.262213112-113
GATCGGA1096100.013.74232116-117
AGATCGG1205400.012.482161110-111
TAGATCG274000.011.719239118-119
CTAGATC70500.011.159989108-109
CACACGT37500.010.243604118-119
TTAGATC99450.010.198894112-113
CACGTCT40400.010.17146120
GAGATCG501850.09.986815114-115
ACGTCTG54850.09.671274120
ACACGTC47800.09.518724118-119
AAGATCG352750.09.33407110-111
Produced by FastQC (version 0.11.5)