FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13637535
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1801300.014.17796120
GCGTCGG65300.013.13769120
TCTCGGG19950.012.629976120
CCCCTTG45150.011.5652961
CCCCTGT46450.011.5000451
CCCCTTT75400.010.427861
CCCCTTA22350.010.2047251
TCATTAA599000.010.165655120
CGTATCA773400.010.161628120
GCCGTAT933600.09.966601120
GTATCAT731500.09.90715120
ATCATTA663950.09.875997120
CGCCGTA1003600.09.791504120
GTCGCCG1169200.09.5848255120
CCGTATC871450.09.472663120
GGTCGCC1241000.09.465359120
CCCCTAA12903.929017E-109.3054091
CATTAAA568000.09.273471120
TCGCCGT1081800.09.216801120
CCCCTGC74950.09.049031
Produced by FastQC (version 0.11.5)