FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13637535
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC219100.0120.798836120
GAGCACA376600.069.491104120
AGAGCAC517600.050.952076118-119
AAGAGCA763100.034.43616120
GAAGAGC882000.029.707085116-117
GGAAGAG1107500.023.698963116-117
TCGGAAG1311800.019.856808114-115
CGGAAGA1327250.019.822088114-115
CACGTCT71850.019.143969120
ATCGGAA1490150.017.760418112-113
CACACGT62900.017.437302118-119
GATCGGA1544700.016.958632112-113
ACACGTC71200.016.667147120
AGATCGG1688700.015.645362110-111
TAGATCG406850.014.196864114-115
GAGATCG523250.013.898817110-111
ACGTCTG80700.013.7025585118-119
CGTCTGA92550.013.599374120
CTAGATC105150.013.46532112-113
GCACACG73850.013.390976118-119
Produced by FastQC (version 0.11.5)