FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14870332
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC171200.082.60772120
GAGCACA284650.051.27635120
AGAGCAC389550.037.174995118-119
AAGAGCA608500.025.298744120
GAAGAGC679600.021.99097118-119
GGAAGAG829950.019.111017120
CGGAAGA973250.016.01903120
TCGGAAG1009500.015.575311118-119
ATCGGAA1192550.013.317252120
GATCGGA1227950.012.839656120
AGATCGG1349350.011.66502118-119
TAGATCG331750.010.953773118-119
TTAGATC139900.010.205702120
CACGTCT44100.09.820181120
CTAGATC83550.09.597814118-119
AAGATCG548300.09.442114114-115
ACACGTC47100.09.194693120
GAGATCG452700.09.043218120
CACACGT37500.08.8422985118-119
CAGATCG413300.08.456785118-119
Produced by FastQC (version 0.11.5)