FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10382934
Sequences flagged as poor quality0
Sequence length50-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT307600.08.484262120
AAGAGTG516300.07.831932120
TGGTCGC177900.07.8238983120
GAGTGTA430600.07.7884126120
AGTGTAG402600.07.7489123120
AGAGTGT468050.07.716412120
GTGTAGA408750.07.705711120
TCTCGGT273650.07.6514044120
AGATCTC316950.07.382186120
GGTGGTC220550.07.2901907120
GTAGATC416500.07.144599120
GTGGTCG204700.07.121961120
ATCTCGG312850.07.0762205120
AAAAAAA326400.06.9111266120
CTCGGTG278100.06.860208120
AGGGAAA744300.06.80308120
TGTAGAT419650.06.790747120
TAGGGAA713550.06.7851634120
TGTAGGG793000.06.7333120
TCGGTGG256500.06.7128305120
Produced by FastQC (version 0.11.5)