FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10382934
Sequences flagged as poor quality0
Sequence length50-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC106050.062.967644120
GAGCACA161150.040.672775120
AGAGCAC221200.032.652973120
AAGAGCA342400.020.810444120
GAAGAGC414850.017.355507118-119
GGAAGAG480600.016.01451120
TCGGAAG547800.013.978517118-119
CGGAAGA568850.013.530058120
ATCGGAA643400.012.49692120
GATCGGA666600.012.285893120
AGATCGG743750.010.95882118-119
TAGATCG166350.010.533038120
CTAGATC41650.010.43902116-117
TTAGATC64600.09.744329120
CACGTCT40750.09.555124120
CCTAGAT13250.09.30573120
GCTAGAT16900.09.023626116-117
AAGATCG320650.07.994265120
CTCTAGA6902.715442E-77.994082114-115
ACACGTC43850.07.9916577120
Produced by FastQC (version 0.11.5)