FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15441911
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC161850.070.50454120
GAGCACA253850.046.225586120
AGAGCAC340850.034.07894118-119
AAGAGCA539450.022.49833120
GAAGAGC601950.019.543518118-119
GGAAGAG746750.016.773872120
TCGGAAG883400.014.134591118-119
CGGAAGA900250.014.060329120
GATCGGA1030000.012.247692118-119
ATCGGAA1045300.012.147123120
AGATCGG1136150.011.1063385116-117
TAGATCG250450.010.4557120
CTAGATC63450.09.979833120
GAGATCG480400.08.945468116-117
CACGTCT53250.08.918596120
AAGATCG399850.08.759536120
TTAGATC96650.08.462718114-115
CAGATCG366450.08.45993120
CACACGT49500.07.52893118-119
CCTAGAT24100.07.5267186112-113
Produced by FastQC (version 0.11.5)