FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11226432
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG60250.011.850808120
AAAAAAA662800.09.523383120
TGGTCGC383250.09.143120
GGTCGCC367350.08.673063120
TCGGTGG538850.08.496003120
GTGGTCG417250.08.354833120
CTCGGTG578100.08.334329120
CGCCGTA318750.08.263659120
ATCTCGG652950.07.9210978120
CGGTGGT520700.07.904867120
TAGATCT764250.07.8588076120
GCCGTAT315350.07.8009777120
TCTCGGT605700.07.74653120
GGTGGTC459200.07.6699805120
CCGTATC301650.07.5983286120
GTCGCCG364750.07.501145120
GATCTCG701400.07.450928120
GTGTAGA961550.07.375706120
CGTATCA258800.07.326235120
AGATCTC735600.07.26765120
Produced by FastQC (version 0.11.5)