FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11226432
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC141500.070.10015120
GAGCACA216100.047.13264120
AGAGCAC285950.035.21765118-119
AAGAGCA432900.023.78712120
GAAGAGC476200.021.426826118-119
GGAAGAG586400.017.531439118-119
CGGAAGA677750.015.2304325118-119
TCGGAAG680950.015.138347116-117
ATCGGAA803000.012.954573120
GATCGGA807850.012.799377114-115
AGATCGG882550.011.660517114-115
TAGATCG224200.011.034299112-113
CTAGATC59700.010.901833112-113
CACACGT36650.010.321241120
AAGATCG315750.09.408606116-117
TTAGATC87750.09.291079108-109
GAGATCG311400.09.236617110-111
CAGATCG291000.08.657658112-113
ACACGTC40550.08.542876118-119
CACGTCT43650.08.096491118-119
Produced by FastQC (version 0.11.5)