FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16105181
Sequences flagged as poor quality0
Sequence length50-126
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC268650.0145.98186120
GAGCACA458050.082.10477120
AGAGCAC632950.060.93501118-119
AAGAGCA933950.040.164486118-119
GAAGAGC1033850.037.171173116-117
GGAAGAG1229800.030.35831116-117
CGGAAGA1424050.027.147865114-115
CACGTCT64050.024.800463120
TCGGAAG1509200.024.794767114-115
ACACGTC61150.022.466331118-119
ATCGGAA1743600.022.30241112-113
CACACGT57900.021.688248120
GATCGGA1796800.020.982002112-113
AGATCGG1944700.020.065731110-111
ACGTCTG79500.019.305725120
TAGATCG524300.017.810778110-111
CTAGATC111200.017.036617108-109
GAGATCG578300.016.834396110-111
CAGATCG653500.016.744999110-111
CGTCTGA93950.015.536718120
Produced by FastQC (version 0.11.5)