FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15139018
Sequences flagged as poor quality0
Sequence length60-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG104550.010.100254120
GTGGTCG329050.09.372272120
TCGGTGG434800.09.369661120
GGTGGTC362700.09.3629465120
TAGATCT632350.09.260532120
AGATCTC602050.09.158547120
CGGTGGT418350.09.078364120
TCTCGGT506750.09.057562120
TGGTCGC299950.09.021345120
CTCGGTG479200.08.977305120
GATCTCG592400.08.7912035120
ATCTCGG563200.08.756949120
AGTGTAG878350.08.559081120
GTGTAGA848850.08.326413120
GTAGATC851550.08.292967120
GGTCGCC305700.08.223604120
GAGTGTA945400.08.205907120
AGAGTGT1017350.08.156342120
AAGAGTG1114250.07.953192120
TGTAGAT879300.07.881131120
Produced by FastQC (version 0.11.5)