FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15139018
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC168550.072.53295120
GAGCACA259450.048.422234120
AGAGCAC356100.036.000607120
AAGAGCA562900.023.41055120
GAAGAGC633700.020.976206120
GGAAGAG752050.017.86379120
CGGAAGA864300.015.532238118-119
TCGGAAG887100.015.046665116-117
ATCGGAA1035150.013.142624116-117
GATCGGA1052700.012.79197116-117
AGATCGG1176350.011.787939120
TAGATCG327150.011.160484114-115
CTAGATC80850.010.026645110-111
TTAGATC133650.09.426801110-111
GAGATCG444600.09.054578118-119
CACACGT46950.08.77566120
AAGATCG397650.08.765627114-115
CACGTCT51400.08.67294120
CAGATCG357400.08.146503112-113
ACACGTC55800.08.110099120
Produced by FastQC (version 0.11.5)