FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14113297
Sequences flagged as poor quality0
Sequence length53-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC309600.09.301441120
GTGGTCG339250.09.054408120
TCGGTGG440000.09.04005120
GGTGGTC366600.08.86986120
CTCGGTG474000.08.75866120
ATCTCGG553350.08.684455120
AGATCTC611000.08.4836445120
CGGTGGT416900.08.447266120
GTGTAGA826900.08.191263120
TCTCGGT505300.08.180501120
TAGATCT638900.08.103785120
GGTCGCC307150.08.076718118-119
AGTGTAG857750.07.777751120
TGTAGAT847500.07.7656326120
GATCTCG599650.07.6637425120
CGTATCA199000.07.56712120
GTAGATC810800.07.5547824120
GCGTCGG89450.07.5118628120
GCCGTAT265350.07.4837427120
GTCGCCG350000.07.3878694120
Produced by FastQC (version 0.11.5)