FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14113297
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC154300.082.79348120
GAGCACA257650.054.006653120
AGAGCAC360350.037.955765120
AAGAGCA554600.025.42011120
GAAGAGC635550.021.905535118-119
GGAAGAG778850.018.58013120
CGGAAGA921950.015.468103120
TCGGAAG945550.015.325987120
ATCGGAA1112200.012.987305118-119
GATCGGA1124500.012.788196116-117
AGATCGG1236250.011.673377116-117
TAGATCG273300.011.096336120
CTAGATC73600.09.863197120
TTAGATC120600.09.6196785120
AAGATCG503300.09.443043114-115
CACACGT29400.09.230482120
GAGATCG433600.09.130148118-119
CAGATCG392900.08.66829120
TAAGATC209050.08.649237114-115
TGAGATC182100.08.308195120
Produced by FastQC (version 0.11.5)