FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13979859
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC172400.067.77693120
GAGCACA263750.043.45496120
AGAGCAC350850.032.94782118-119
AAGAGCA533300.021.949553120
GAAGAGC580450.019.921152118-119
GGAAGAG696700.016.641233120
TCGGAAG784200.014.678467114-115
CGGAAGA793600.014.618952114-115
CACACGT49050.013.527197120
ACACGTC50300.013.468741120
ATCGGAA926150.012.872873120
GATCGGA925350.012.579156112-113
CACGTCT52600.011.55199120
AGATCGG1028400.011.400125112-113
TAGATCG180800.010.655987110-111
CTAGATC49650.09.630453108-109
AAGATCG507050.09.554227114-115
TTAGATC83500.08.694283114-115
ACGTCTG64150.08.492219120
GAGATCG344650.08.476181110-111
Produced by FastQC (version 0.11.5)