FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12199197
Sequences flagged as poor quality0
Sequence length58-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG33600.09.820596120
AAAAAAA575850.09.751712120
CGCCGTA274200.08.642593120
GTCGCCG318600.08.436196120
GTGGTCG387900.08.413827120
CGTATCA245450.08.286118120
GCCGTAT282050.08.1468120
TCGGTGG501500.08.08705120
GGTGGTC421400.08.072404120
GGTCGCC349500.07.9134912120
CCGTATC273100.07.9085193120
CTAGGCC3500.0073412917.713979264-65
TGGTCGC361650.07.572978118-119
CTCGGTG535800.07.5357547120
GATCTCG608600.07.314529120
TCGCCGT326450.07.305246118-119
TCATTAA205700.07.291554120
GTCTGCG70000.07.2852161
TCTCGGT587100.07.2758265120
CGGTGGT472050.07.1363506118-119
Produced by FastQC (version 0.11.5)