FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13842251
Sequences flagged as poor quality0
Sequence length72-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG69100.09.726467120
AGATCTC401950.09.599621120
TAGATCT414700.09.521536120
GTGGTCG213800.09.234285120
CTCGGTG303550.08.994899120
GAGTGTA611500.08.76335120
GATCTCG377100.08.720455120
GTGTAGA553700.08.691905120
TCGGTGG293200.08.677948120
ATCTCGG352500.08.631057120
TGGTCGC199300.08.370523120
TCTCGGT317850.08.286111118-119
GGTGGTC247950.08.27708120
AAGAGTG736900.08.192276120
AGTGTAG566650.08.08025120
GTAGATC564800.07.947345120
AGAGTGT664750.07.7544317120
TGTAGAT594100.07.525093120
GTGTAGG1248000.07.116431120
TAGGGAA1060100.07.03622120
Produced by FastQC (version 0.11.5)