FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16679561
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC209250.077.73376120
GAGCACA332750.051.294083120
AGAGCAC452450.037.799522120
AAGAGCA688150.024.930141118-119
GAAGAGC754650.022.406778118-119
GGAAGAG938650.018.512426120
CGGAAGA1142950.015.599353120
TCGGAAG1146100.015.448779120
ATCGGAA1381700.013.320766120
GATCGGA1378800.013.062979118-119
AGATCGG1499750.012.016194120
TAGATCG291300.011.393714120
CTAGATC71900.010.138272116-117
AAGATCG668900.09.864137116-117
GAGATCG480950.09.627446116-117
TTAGATC124750.09.409438118-119
CAGATCG468850.08.744586114-115
TAAGATC279800.08.34167114-115
TGAGATC233950.08.060651120
CACGTCT47400.07.6675744120
Produced by FastQC (version 0.11.5)