FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13209739
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG372150.09.675511120
TAGATCT257550.09.662821120
GAGTGTA411000.09.404469120
ATCTCGG239650.09.356135120
TCGGTGG206750.09.129548120
AGATCTC252200.08.962061120
AGAGTGT453100.08.955191120
GTGTAGA369300.08.757257120
GCGTCGG62050.08.525228120
TCTCGGT223400.08.449122120
GATCTCG248600.08.390608120
GTAGATC363750.08.130687120
AAGAGTG514100.08.091399120
CTCGGTG207500.07.9377537120
TAGGGAA738800.07.9005523120
TGTAGAT412700.07.763506120
GTAGGGA771250.07.6460814120
GTGGTCG149850.07.5817623120
TGTAGGG820800.07.5360394120
GTGTAGG888550.07.489121120
Produced by FastQC (version 0.11.5)