FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13209739
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC127050.068.314865120
GAGCACA205950.040.7062120
AGAGCAC266700.032.180298118-119
AAGAGCA433350.021.33516120
GAAGAGC485300.019.130838120
GGAAGAG606250.016.110088120
TCGGAAG692100.013.960006118-119
CGGAAGA707900.013.684724118-119
GATCGGA803000.012.547412120
ATCGGAA806100.012.163933120
AGATCGG887350.011.21691120
TAGATCG244450.010.449089120
CTAGATC60100.010.384252120
TTAGATC92000.09.60111118-119
GAGATCG309150.09.198795120
AAGATCG331850.08.807977118-119
CAGATCG273850.08.104793118-119
ATAGATC121900.07.442063120
CCTAGAT23750.07.1678486118-119
TAAGATC124250.06.9388313120
Produced by FastQC (version 0.11.5)