FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14135158
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC216450.0118.156586120
GAGCACA367550.070.2569120
AGAGCAC504300.051.942932120
AAGAGCA725700.036.090687118-119
GAAGAGC828000.030.992458118-119
GGAAGAG1005900.025.985336116-117
CGGAAGA1165950.022.03801116-117
TCGGAAG1191550.021.804626114-115
CACACGT57750.020.640242120
CACGTCT63250.019.900782120
ATCGGAA1384800.018.765217114-115
GATCGGA1427300.018.410845112-113
ACACGTC63450.017.583727120
AGATCGG1562550.016.67383112-113
TAGATCG320550.015.648188110-111
CGTCTGA93100.014.441959120
GCACACG78800.014.158471120
CTAGATC67050.014.151367110-111
GAGATCG558900.013.999696110-111
ACGTCTG79550.013.545499120
Produced by FastQC (version 0.11.5)