FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13592778
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC122100.060.847717120
GAGCACA186700.042.09028120
AGAGCAC244900.031.278143118-119
AAGAGCA396250.019.928814118-119
GAAGAGC427000.018.328474120
GGAAGAG546350.014.921971120
TCGGAAG620500.013.901955120
CGGAAGA652950.013.2110615120
GATCGGA716650.012.028073118-119
ATCGGAA727500.011.94523120
AGATCGG794150.011.402017120
TAGATCG199450.010.331172120
CTAGATC55650.09.429953114-115
GAGATCG322750.08.664645118-119
AAGATCG314600.08.644859120
TTAGATC86550.08.318041118-119
CCTAGAT22250.08.052964120
TAAGATC123900.07.334198118-119
CAGATCG259350.07.106386116-117
CACACGT41200.06.989451120
Produced by FastQC (version 0.11.5)