FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14813203
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC197200.066.06788120
GAGCACA297800.044.048786120
AGAGCAC376100.034.579792118-119
AAGAGCA549100.023.964436120
GAAGAGC606800.021.280329116-117
GGAAGAG725150.017.693897118-119
CGGAAGA847600.015.396038114-115
TCGGAAG861500.014.865276114-115
ATCGGAA1012050.013.246114120
GATCGGA1054750.012.63185120
CACGTCT60400.012.488188120
CACACGT57900.011.716183118-119
AGATCGG1188550.011.426565118-119
TAGATCG274850.010.882234118-119
ACACGTC71250.010.778959120
CTAGATC78450.09.781081108-109
TTAGATC107650.08.911314116-117
AAGATCG416150.08.733125120
GAGATCG460550.08.547572118-119
CGTCTGA78900.08.343301120
Produced by FastQC (version 0.11.5)