FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14986649
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC185650.085.7863120
GAGCACA301750.051.02867120
AGAGCAC415950.038.522778120
AAGAGCA630250.025.777075120
GAAGAGC705400.023.159021120
GGAAGAG841850.019.367458118-119
CGGAAGA983200.016.792273120
TCGGAAG1033950.016.23026120
ATCGGAA1203000.013.954794118-119
GATCGGA1245850.013.324665120
AGATCGG1385150.012.079513116-117
TAGATCG303650.011.332342120
CTAGATC76150.010.58951114-115
AAGATCG497300.09.882999114-115
GAGATCG531700.09.694299118-119
TTAGATC136650.09.462177120
CAGATCG427000.08.5059595112-113
TAAGATC204350.08.4875765114-115
CACACGT35050.08.329773118-119
ACACGTC46200.08.275771120
Produced by FastQC (version 0.11.5)