FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12348862
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC141150.061.459454120
GAGCACA210550.041.107914120
AGAGCAC275750.032.43756120
AAGAGCA432150.022.006884120
GAAGAGC462200.020.67571120
GGAAGAG556200.017.077238118-119
CGGAAGA645900.015.0499115120
TCGGAAG659550.014.628748120
ATCGGAA797050.012.806515120
GATCGGA804400.012.689498120
CACACGT37500.012.27698120
AGATCGG906000.011.381319118-119
CACGTCT38950.010.806804120
CTAGATC51350.010.502633120
TAGATCG179700.010.467495120
ACACGTC43300.010.024918120
AAGATCG397750.09.259822120
GAGATCG335650.08.9939575120
TTAGATC81950.08.828126120
ACGTCTG50500.08.335149120
Produced by FastQC (version 0.11.5)