FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11971228
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC103450.056.55945120
GAGCACA154400.038.963043120
AGAGCAC204350.031.114315120
AAGAGCA329200.019.71851120
GAAGAGC372750.018.571156120
GGAAGAG476150.015.377018120
TCGGAAG551250.014.616079120
CGGAAGA575800.012.991051120
GATCGGA640850.012.483517120
ATCGGAA649900.012.007546118-119
AGATCGG709100.011.255174120
TAGATCG180700.011.190938120
CTAGATC47900.09.926113114-115
TTAGATC76650.09.59356120
AAGATCG279650.09.135329120
GAGATCG289100.08.902498120
CCTAGAT17350.08.403541120
CAGATCG260800.07.243403120
GCTAGAT18950.06.8578715112-113
TCTAGAT27150.06.771312112-113
Produced by FastQC (version 0.11.5)