FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12678945
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC156750.083.32007120
GAGCACA265900.051.61266120
AGAGCAC359500.037.674934120
AAGAGCA556800.024.738983118-119
GAAGAGC642250.022.551867120
GGAAGAG771300.018.491907120
CGGAAGA907000.016.121437120
TCGGAAG928600.015.505869118-119
GATCGGA1101400.013.777887120
ATCGGAA1073150.013.552219118-119
AGATCGG1212950.012.231867118-119
TAGATCG329250.011.585336120
CTAGATC78550.010.468781120
GAGATCG416500.010.386152120
AAGATCG348350.010.194964118-119
CAGATCG377100.09.765222114-115
TTAGATC109450.09.577428108-109
ACACGTC51950.09.311247120
CCTAGAT30900.08.926864114-115
TGAGATC155900.08.732035120
Produced by FastQC (version 0.11.5)