FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15663961
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC252250.0122.6072120
GAGCACA415700.070.03829120
AGAGCAC579900.052.128773118-119
AAGAGCA850650.034.81389118-119
GAAGAGC981300.030.829365116-117
GGAAGAG1154850.025.551655116-117
CGGAAGA1345950.022.459518114-115
TCGGAAG1409950.020.905437114-115
ATCGGAA1624400.018.678535112-113
GATCGGA1673650.017.65363112-113
CACACGT55500.016.997618120
AGATCGG1814250.016.721966110-111
CACGTCT61550.016.378696120
TAGATCG433300.015.412005110-111
CTAGATC122800.014.649806108-109
GAGATCG642950.013.7163725112-113
CAGATCG603300.013.69033112-113
AAGATCG503950.013.086176110-111
TTAGATC160500.012.851106110-111
ACACGTC61650.012.751696118-119
Produced by FastQC (version 0.11.5)