FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13494175
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC125100.057.38746120
GAGCACA185700.040.51573120
AGAGCAC243700.032.522797120
AAGAGCA401150.019.53054118-119
GAAGAGC456000.017.40914120
GGAAGAG588050.013.944748120
TCGGAAG637600.013.281416120
CGGAAGA685500.012.04616120
GATCGGA759100.011.702052120
ATCGGAA765800.011.674668120
AGATCGG821400.011.226254120
TAGATCG173700.010.213316120
CTAGATC49900.09.719296120
AAGATCG371600.08.65519120
TTAGATC71150.08.639392116-117
GAGATCG306150.08.275191120
ACACGTC58150.07.791668120
CCTAGAT17450.07.679724120
CCCTAGA8700.00357707087.335041120
CAGATCG322150.07.210494120
Produced by FastQC (version 0.11.5)