FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13391133
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC160500.078.52929120
GAGCACA248850.049.137295120
AGAGCAC337850.036.71933118-119
AAGAGCA529300.024.80737120
GAAGAGC583150.021.795341118-119
GGAAGAG732150.017.812792120
TCGGAAG851300.015.227285118-119
CGGAAGA864500.015.085755120
GATCGGA1031700.012.661029116-117
ATCGGAA1013000.012.648504114-115
AGATCGG1110800.011.519657112-113
TAGATCG222750.010.853419114-115
CTAGATC59500.010.574643114-115
AAGATCG551500.09.474351112-113
TTAGATC101200.09.393245120
GAGATCG369500.08.828466120
CAGATCG335300.07.771168114-115
CCTAGAT19200.07.479963120
TAAGATC246150.07.2375116118-119
ACACGTC35200.07.091519116-117
Produced by FastQC (version 0.11.5)