FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13541705
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC152200.073.98358120
GAGCACA241900.044.352627120
AGAGCAC324800.036.22166120
AAGAGCA504300.023.74245120
GAAGAGC571550.020.836468118-119
GGAAGAG689850.017.873201120
CGGAAGA813750.014.953055118-119
TCGGAAG816250.014.899434120
ATCGGAA959950.012.72134116-117
GATCGGA978550.012.558856120
AGATCGG1074800.011.343126118-119
TAGATCG245000.010.638687118-119
TTAGATC101300.09.661371116-117
CTAGATC64550.09.170284112-113
AAGATCG426700.09.025431114-115
GAGATCG417050.08.79816116-117
CAGATCG331250.07.848358116-117
CACGTCT45050.07.390823118-119
TGAGATC169850.07.3860226110-111
TAAGATC161500.07.3097243112-113
Produced by FastQC (version 0.11.5)