FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14219514
Sequences flagged as poor quality0
Sequence length68-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG84900.010.051674120
ATCTCGG300950.09.525369120
GATCTCG324150.09.511064120
GAGTGTA522150.09.414902120
GTGTAGA470850.09.355762120
TAGATCT340200.09.239003120
GTAGATC466200.09.152587120
AGATCTC342400.09.091882120
AGTGTAG482600.09.078162120
TCTCGGT275300.08.950255120
AGAGTGT571200.08.922066120
CTCGGTG273900.08.315817120
TGGTCGC168350.08.139158120
GGTCGCC178650.08.107217120
GTAGGGA967450.07.920259120
AAGAGTG637700.07.8762355118-119
TAGGGAA916800.07.7219753120
TGTAGAT505800.07.663702120
TCGGTGG251400.07.6018553120
GTGGTCG187700.07.5289826118-119
Produced by FastQC (version 0.11.5)