FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14219514
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC148850.067.015015120
GAGCACA224600.046.05591120
AGAGCAC307650.033.121735118-119
AAGAGCA489950.021.812912120
GAAGAGC556500.019.320696120
GGAAGAG690650.015.989665120
TCGGAAG785150.014.304254120
CGGAAGA806100.014.012796120
ATCGGAA935450.012.157512118-119
GATCGGA941550.011.944883118-119
AGATCGG1031500.011.164134120
TAGATCG239000.010.562946120
CTAGATC62150.09.775996112-113
TTAGATC109900.09.424117120
AAGATCG460100.08.759132114-115
GAGATCG358850.08.555721118-119
ACACGTC42500.08.224758120
CACACGT40350.07.7691627118-119
TCTAGAT34150.07.664599110-111
CAGATCG320250.07.4980626116-117
Produced by FastQC (version 0.11.5)