FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12822746
Sequences flagged as poor quality0
Sequence length52-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA2667850.015.424712120
TCATTAA955500.010.366546120
CATTAAA908300.010.363616120
CGTATCA1260150.010.350348120
TCTCGGG21450.010.34885120
GTATCAT1197000.010.189689120
CCGTATC1384600.09.987672120
ATCATTA1057150.09.840795120
GCCGTAT1500750.09.670417120
TATCATT1217650.09.425625120
CGCCGTA1611750.09.324546120
ATTAAAA1048550.08.794322120
CGGTGGG19850.08.765065120
TCGCCGT1769450.08.727461120
GGTCGCC1999350.08.576124120
GCGTCGG65100.08.570766120
CCCCGTG12903.639616E-88.3718051
GTCGCCG1888800.08.36657120
GTGGTCG2293400.08.018043120
TGGTCGC2139350.07.9447894120
Produced by FastQC (version 0.11.5)