FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12822746
Sequences flagged as poor quality0
Sequence length50-126
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC192850.0136.18571120
GAGCACA331300.078.376625120
AGAGCAC445400.058.4217118-119
AAGAGCA670050.038.296406118-119
GAAGAGC729300.035.527233116-117
GGAAGAG884450.028.791521116-117
CGGAAGA1020200.025.43348114-115
CACACGT46650.024.804264120
TCGGAAG1066750.023.791834114-115
CACGTCT56250.021.703354120
ATCGGAA1256200.020.96335112-113
ACGTCTG69750.020.242256120
GATCGGA1273900.020.16849112-113
ACACGTC53350.019.500383120
AGATCGG1375050.019.2054110-111
TAGATCG328700.016.871641110-111
GCACACG59200.016.31816120
CGTCTGA80100.016.30137120
GAGATCG432250.015.749085110-111
CTAGATC73800.015.5893755116-117
Produced by FastQC (version 0.11.5)