FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15005615
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC119700.047.744892120
GAGCACA165200.035.163185120
AGAGCAC210000.029.688643120
AAGAGCA390000.017.751734120
GAAGAGC431200.016.070122120
TCGGAAG591050.012.401749120
GGAAGAG585650.012.355774120
GATCGGA751700.011.22524120
ATCGGAA743250.011.159154120
CGGAAGA682800.011.07449120
AGATCGG776550.010.801023118-119
TAGATCG167550.010.049817120
CTAGATC45850.09.810998118-119
TTAGATC78950.07.2943397120
AAGATCG401200.07.099058120
CCTAGAT17300.07.0422025118-119
GAGATCG331800.06.753936120
ACACGTC54900.06.499093120
CAGATCG342650.06.354364118-119
TCTAGAT28350.06.1823735120
Produced by FastQC (version 0.11.5)