FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12975416
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC173700.091.12341120
GAGCACA282600.052.43559120
AGAGCAC382800.040.010185118-119
AAGAGCA578550.026.8903120
GAAGAGC656600.023.376698116-117
GGAAGAG792450.019.51196118-119
CGGAAGA925750.016.675205114-115
TCGGAAG945650.016.304827116-117
ATCGGAA1092850.014.149059112-113
GATCGGA1141500.013.590809114-115
AGATCGG1258300.012.317722110-111
TAGATCG279700.011.485698114-115
CACACGT44000.011.47545120
CACGTCT48750.011.408072120
ACGTCTG58650.011.353941120
CTAGATC57350.010.335343118-119
GAGATCG515100.010.264432112-113
ACACGTC54950.09.987731120
AAGATCG396550.09.799078112-113
TTAGATC100750.09.406039116-117
Produced by FastQC (version 0.11.5)