FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11912056
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC125950.061.18432120
GAGCACA182200.040.704506120
AGAGCAC244050.031.225328120
AAGAGCA382600.021.019539120
GAAGAGC420250.018.885643120
GGAAGAG510900.015.753888118-119
TCGGAAG595400.013.772506120
CGGAAGA612200.013.383802120
GATCGGA684400.012.5685625120
ATCGGAA701000.011.819934118-119
AGATCGG745450.011.181397118-119
CTAGATC49350.010.944077120
TAGATCG184450.010.855878114-115
TTAGATC74450.010.041679116-117
AAGATCG288250.08.511565118-119
TCTAGAT22150.08.47509112-113
CAGATCG283200.08.302855120
GAGATCG250050.08.086563120
GCTAGAT22450.07.481287118-119
CCTAGAT16300.07.4754367118-119
Produced by FastQC (version 0.11.5)