FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15626909
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC135750.064.56158120
GAGCACA205800.042.742737120
AGAGCAC278450.034.159706120
AAGAGCA459200.020.896225120
GAAGAGC490350.019.432741118-119
GGAAGAG650500.014.742393118-119
TCGGAAG741150.012.99031120
CGGAAGA789550.012.373933116-117
TAGATCG242350.011.753149120
GATCGGA840700.011.735704120
ATCGGAA872750.011.378573120
AGATCGG912200.010.833674118-119
CTAGATC58900.09.824698118-119
TTAGATC103950.09.0616045118-119
GAGATCG292350.08.927441120
AAGATCG404350.08.610592118-119
CAGATCG283650.07.4291654120
TGAGATC134350.06.8112326120
GCTAGAT27350.06.597444120
TAAGATC159750.06.520382114-115
Produced by FastQC (version 0.11.5)