FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11900327
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC133550.067.86085120
GAGCACA205250.044.640232120
AGAGCAC274600.033.245487120
AAGAGCA415600.022.89292120
GAAGAGC465400.020.036974118-119
GGAAGAG568150.017.423891120
TCGGAAG673400.014.385095120
CGGAAGA680650.014.06137118-119
ATCGGAA792650.012.388457120
GATCGGA798850.012.234307118-119
AGATCGG895550.011.454316120
CACACGT39850.010.829679120
TAGATCG227450.010.77137120
CTAGATC51950.010.544022114-115
TTAGATC79150.08.640206114-115
GAGATCG368650.08.536914118-119
CACGTCT43650.08.517935120
CCTAGAT18500.08.25456114-115
AAGATCG287150.08.138875120
ACACGTC45800.08.118075120
Produced by FastQC (version 0.11.5)