FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15509980
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC185000.073.19295120
GAGCACA281850.047.120182120
AGAGCAC383000.035.045826118-119
AAGAGCA594600.022.66585118-119
GAAGAGC620800.021.324745118-119
GGAAGAG780800.017.307045120
TCGGAAG913900.014.649797118-119
CGGAAGA924400.014.529722120
GATCGGA1130150.012.289936120
ATCGGAA1126550.012.2867155120
AGATCGG1217000.011.43272118-119
ACACGTC59350.011.177039120
TAGATCG277150.010.667397114-115
CACGTCT55050.010.283259118-119
CTAGATC71500.09.824819116-117
AAGATCG576600.09.344083114-115
CACACGT53400.09.196048118-119
TTAGATC131450.08.586789116-117
GAGATCG371400.08.501799110-111
ACGTCTG77100.08.426456120
Produced by FastQC (version 0.11.5)