FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13122676
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC121300.065.12216120
GAGCACA188450.045.16539120
AGAGCAC245550.033.665558120
AAGAGCA395000.022.33086120
GAAGAGC455050.019.341522120
GGAAGAG590500.016.0288120
TCGGAAG672450.014.075405120
CGGAAGA692700.013.440696120
ATCGGAA789300.012.628371120
GATCGGA798050.012.190099118-119
AGATCGG873150.011.297582120
CTAGATC59900.010.308284118-119
TAGATCG254550.010.301973120
CAGATCG349300.08.8356120
GAGATCG292900.08.535155120
TTAGATC96600.08.404125120
AAGATCG314300.08.077016120
ACACGTC47000.07.8140483120
CACACGT43600.06.6500535120
CCTAGAT26650.06.637848112-113
Produced by FastQC (version 0.11.5)