FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12221209
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC121550.056.225155120
GAGCACA174100.040.627056120
AGAGCAC232250.031.53968120
AAGAGCA371800.021.108963120
GAAGAGC402600.018.715994118-119
GGAAGAG520250.015.433308120
TCGGAAG587650.013.641275118-119
CGGAAGA616800.013.300236120
GATCGGA711950.012.402779120
ATCGGAA722150.012.343879120
AGATCGG782050.011.381948118-119
CTAGATC55400.010.843592120
CACGTCT46800.010.76586120
TAGATCG182650.010.203004118-119
CACACGT51200.09.462182120
ACACGTC49700.08.83797120
TTAGATC76000.08.499401116-117
GAGATCG278900.08.152599118-119
GCACACG65200.08.123931120
AAGATCG335000.08.098535118-119
Produced by FastQC (version 0.11.5)